Cardiac stem/progenitor cells, secreted proteins, and proteomics

Disulfide proteomics for identification of extracellular or secreted proteins.

The combination of SDS-PAGE and MS is one of the most powerful and perhaps most frequently used gel-based proteomics approaches in protein identification. However, one drawback of this method is that separation takes place under denaturing and reducing (R) conditions and as a consequence, all proteins with identical apparent molecular mass (Mr) will run together. Therefore, low-abundant protein...

Proteins secreted by human trabecular cells. Glucocorticoid and other effects.

The capacity of cultured human trabecular meshwork (HTM) cells to secrete an extracellular matrix was studied by indirect immunofluorescence. Synthesis of nine extracellular matrix (ECM) proteins known to be present in the normal trabecular meshwork was assessed in three HTM cell lines. Fourteen primary antibodies were used and cultures were labeled two and four weeks after confluence. The HTM ...

Cardiac Proteomics

Approximately one-third of all deaths are attributed to cardiovascular diseases, and ischaemic heart disease is the primary cause of death globally. Hence there continues to be a pressing need for advances in preventative, diagnostic, and interventional strategies for cardiovascular disease. The mul-tifactorial nature of cardiovascular disease, which encompasses both genetic and environmental f...

Secreted Immunomodulatory Viral Proteins

Ubiquitinated Proteins in Exosomes Secreted by Myeloid-Derived Suppressor Cells

We provide evidence at the molecular level that ubiquitinated proteins are present in exosomes shed by myeloid-derived suppressor cells (MDSC). Ubiquitin was selected as a post-translational modification of interest because it is known to play a determinant role in the endosomal trafficking that culminates in exosome release. Enrichment was achieved by two immunoprecipitations, first at the pro...